What is polygalacturonase?

Polygalacturonase is extracted from the digestive juice of snails. It can mainly decompose pectin or pectinase and is often used in juice clarification. However, many people are not very familiar with the substance called polygalactose whole sour plum, so the editor has compiled a lot of content about this enzyme. Let’s take a closer look at the explanation of this substance and enrich your knowledge.

Polygalacturonase (EC 3.2.1.15, abbreviated as PG or endo-PG) is the common name of poly-α-1,4-galacturonide glycanohydrase, which is a type of enzyme that can decompose pectin or pectin acid, also known as pectinase or pectin depolymerase. It is an enzyme that hydrolyzes the α-1,4-glycosidic bond of D-galacturonic acid that makes up pectin acid. It is of great significance in the food industry, especially in fruit juice clarification.

In addition to being found in higher plants, molds and bacteria, PG is also found in the digestive juices of snails. The enzyme activity is determined using pectin acid as a substrate to check whether the reducing ability of the reaction solution increases or the viscosity decreases under certain conditions. Various types of enzymes are known, and they can be divided into endo-type and exo-type according to the manner in which they cleave substrate molecular chains. In addition, the enzyme is selective for the degree of esterification of the substrate and uses pectin acid with a high ester content as a substrate, which is also called polymethylgalacturonase.

PG is a cell wall structural protein that can catalyze the cleavage of poly-α-(1,4)-galacturonic acid in pectin molecules, participate in the degradation of pectin, disintegrate the cell wall structure, and cause the fruit to soften. The members of the PG multigene family are expressed in different stages and tissues of plant development. PG is related to fruit ripening, cell separation processes (such as leaf and flower abscission, pod cracking, pollen ripening, pathogen defense, plant-host interaction), and cell extension, development and lignification. Therefore, PG has always been a hot topic for research on plant development and fruit ripening and aging.

The above is the content about polygalacturonase compiled by the editor. Have you learned all this knowledge? Have you learned about it? Galactogalacturonase can also inhibit the structure of protein and is mainly found in plants. It is mostly used when plant cells divide to promote faster growth and faster fruiting.