What are the methods for detecting chloramphenicol?

When it comes to chloramphenicol, many people may not know about it. This is actually what people commonly call antibiotics. Chloramphenicol may be used in the treatment of many diseases. Some diseases require professional medical examinations, and some medical examinations also require chloramphenicol testing. There are many methods for detecting chloramphenicol clinically. Too much chloramphenicol in the body can have a great impact on health, so you need to pay attention to the intake. So what exactly is the chloramphenicol test?

1. Chloramphenicol detection

Chloramphenicol is a broad-spectrum antibiotic that was once widely used in animal husbandry. However, chloramphenicol has strong side effects and toxic effects. If chloramphenicol remains in edible animals, it can be transmitted to humans through the food chain. Long-term trace intake of chloramphenicol will not only cause the normal intestinal flora of the human body to be disturbed, but also cause a variety of diseases, posing a threat to human health. Many countries in the world prohibit the use of this drug in food-producing animals and stipulate that chloramphenicol must not be detected in livestock products.

2. Main methods

Chloramphenicol detection methods include microbiological method, chromatography, and immunoassay. The microbiological method is easy to operate and low in cost, but has low sensitivity and poor specificity. The chromatography method is accurate, reliable and highly sensitive, with a detection limit of 0.1 μg/kg, but it has many pretreatment steps and a low recovery rate. Immunoassay has the advantages of high sensitivity, strong specificity, low requirements for instruments and personnel quality, and simple sample pretreatment. It is suitable for on-site monitoring and large-scale sample screening.

3. Reaction Principle

ELISA method: Chloramphenicol enzyme-linked immunosorbent assay kit is mainly based on the basic principle of specific immunochemical reaction between antigen and antibody. In other words, it uses enzyme markers to compete with chloramphenicol in the sample and antibodies in the microwells. During the entire reaction, if there is more chloramphenicol residue in the sample, there will be more enzyme markers that compete for the reaction, resulting in a relatively reduced amount of enzyme markers that can bind to the antibody. The chloramphenicol content in the sample is inversely proportional to the absorbance value of the sample when using TMB substrate for color development. The chloramphenicol content can be obtained by comparing with the standard curve.