Rubella virus antibody igm

Rubella is an acute infectious disease that easily occurs in spring. Not only infants and young children are susceptible to the disease, but if pregnant women become ill, it may cause fetal malformations. Therefore, when a suspected illness is discovered, it is necessary to conduct rubella virus antibody igm testing to determine the physical condition based on whether the test result is negative or positive. Let's take a closer look at the details of rubella virus antibody igm.

Rubella is common in school-age children and adolescents, and more than 80% of the population is positive for antibodies to the virus. If a pregnant woman is infected with rubella virus before 20 weeks of pregnancy, the incidence of fetal teratogenesis is higher. Rubella virus infection causes a rash in adults and children.

How to operate

(1) Sample collection: For screening tests, only a single blood sample is required. However, if the immune status of a person infected with the virus needs to be determined, samples must be taken from suspected rubella patients within 3 days after the onset of the rash and within the following 14 to 21 days for simultaneous testing.

(2) As with general ELISA, add 50 μl of PBS to each well of the control and sample wells, followed by 10 μl of the sample, incubate at 25°C for 45 min, wash, and dry.

(3) Add 250 μl of enzyme marker to each well and keep warm and wash in the same way.

(4) Add 250 μl of pNPP substrate solution, keep warm and wash in the same way, then add 50 μl of 1 mol/L sodium hydroxide to terminate the reaction. Measure the absorbance of each well at 405 nm to determine the results of the tested samples.

(5) If the result is positive, the sample can be further diluted and the antibody titer can be measured. The results of the two samples can be compared and judged.

determination

1. Both IgG and IgM antibodies to rubella virus are positive, or the IgG antibody titer is ≥1:512, indicating recent infection with rubella virus.

2. Both IgG and IgM antibodies of Rubella virus are negative, indicating that there has been no Rubella virus infection.

3. The IgG antibody titer of rubella virus is <1:512, and the IgM antibody is negative, indicating a history of infection.

4. In addition, reinfection with rubella virus is difficult to detect because IgM antibodies only appear briefly or at very low levels. Therefore, if the rubella virus IgG antibody titer is increased by more than 4 times in the duplicate sera, it is an indicator of recent rubella virus infection regardless of whether the IgM antibody is positive.