When is the best time to check for mycoplasma

Many people are infected with mycoplasma due to unhealthy sexual life, contact transmission or infection from other people's underwear. These are the main causes of mycoplasma infection. When the disease is examined, if you find some discomfort or are more sensitive, you should go to the hospital for examination as soon as possible, because this examination is not shameful, but can detect it early and allow the body to recover quickly.

Clinical diagnosis

For patients with a history of unclean sexual intercourse, contact infection, urethral and vaginal secretions, and burning pain during urination, but without the possibility of infection by other pathogens, a smear of urethral or cervical secretions can be taken for a preliminary diagnosis.

Laboratory diagnosis

1. Mycoplasma culture:

a. Collect specimens, usually urogenital swabs or swabs, and a few take prostatic fluid, semen, joint fluid, or fallopian tube, rectal biopsy, and use first-pass urine specimen centrifuge to replace urethral swabs. When taking the swab, insert the swab 2-4cm into the male urethra and rub it vigorously. This method can easily cause urethral injury and secondary infection. Be careful when using. Women need to first clean the junction of the squamous and columnar epithelium of the cervix and collect cervical specimens with a cell brush, which can increase the number of infected cells and is more sensitive than a cotton swab.

b. The commonly used culture medium is ox heart extract or peptone, containing 1% fresh yeast extract, 10-20% animal serum and 0.5% sodium chloride. Glucose and arginine can also be added to promote the growth of MH and MG, urea can be added for UU metabolism, and penicillin can be added in appropriate amounts to inhibit miscellaneous bacteria.

2. Serological identification method: The most commonly used method is agar diffusion method, which is to inoculate mycoplasma onto an agar dish. Then place filter paper soaked in an appropriate amount of serum on the agar surface to observe which one can inhibit the growth of mycoplasma. The colonies on the agar surface can also be stained with fluorescent antibodies and observed under an epifluorescence microscope. The advantage of this method is that the colonies originally grown on the agar surface can be used without the need to subculture the mycoplasma.

Serological diagnostic tests: Enzyme-linked immunosorbent assay (ELISA) has high sensitivity; microimmunofluorescence assay (MIF) has rapid characteristics.

3. Genetic diagnosis: The sensitivity of using DNA probes to diagnose mycoplasma is slightly lower, but the specificity is high. The polymerase chain reaction (PCR) has high sensitivity and specificity.

Principles of diagnosis and treatment

1. Those who have a relatively typical infection process, regardless of whether they have symptoms or not (because about half of them have asymptomatic symptoms, and by the time they become obvious, damage may have been caused), those who test positive for chlamydia, regardless of gender, need treatment;

2. Some people who have no symptoms of urethritis are accidentally diagnosed during physical examinations, or some women are found to be infected with mycoplasma after pregnancy but have no signs of inflammation during local examinations. They are most likely carriers and there is some disagreement on whether to treat them. For ordinary people, no special treatment is needed, or after regular use of antibiotics, it can turn negative, but there is no need to force it if it does not turn negative; for pregnant women, it is generally recommended to use medication to turn negative, but there is no need to be too demanding.

3. In terms of medication, antibiotic resistance is becoming more and more obvious. The types of resistance and drugs in each region are not the same. Therefore, some people prefer to use minocycline, while some people prefer to use azithromycin. A study in Guangdong showed that josamycin is most sensitive to ureaplasma urealyticum; but because of this, when a certain antibiotic is widely used, the chance of its resistance is greatly increased. Combination therapy is sometimes a last resort.

4. If the empirical medication is not effective after one course of treatment, drug sensitivity testing should be actively performed.

5. The combination of Chinese and Western medicine can not only improve symptoms more quickly, but also reduce drug resistance to a certain extent. Of course, this combination is not Chinese medicine plus Western medicine, but an organic combination.

6. The Chinese medicine Fuyan Pill can be used to treat mycoplasma by promoting blood circulation and removing blood stasis, strengthening the spleen and removing dampness.