What is the formula for the anticoagulant formula?

Anticoagulants are blood that cannot clot. When human blood is separated from the body, it will coagulate. This is a normal phenomenon. Serum will gradually appear. The test requires that the blood cannot coagulate, otherwise the coagulated blood cannot be tested. At this time, the blood must not coagulate. At this time, an anticoagulant is needed to prevent the blood from coagulating. What is the formula of the anticoagulant formula? Let’s take a look below.

1. Prepare the ingredients

Animals, syringes, sodium citrate, etc.

2. Steps

1. Preparation of anticoagulant

There are many kinds of anticoagulants, such as heparin, sodium citrate, sodium citrate, EDTA, etc., each of which has its own suitable application. Generally speaking, sodium citrate (3%-5%) is the most commonly used and simple formula in laboratories. The ratio to blood is 1:4, which is 2ml anticoagulant + 8ml blood.

2. Animal Restraint

Taking chicken as an example, fresh chicken anticoagulated blood was collected using a 10ml fine needle syringe from the wing root vein. Grasp the chicken's wings with your left hand and make it lie on its side with its body facing inward and its wings facing outward to complete the restraint.

3. Blood collection

Take an alcohol cotton ball in your right hand and wipe it along the chicken's armpit toward the outside of the wing. An obvious vein will appear, which is the wing root vein. Press the proximal end with your left hand, hold a 10ml syringe with 2ml of anticoagulant in your right hand and insert it parallel to the vein. Release your left hand and 8ml of blood can be collected.

4. Stop bleeding

After blood collection, carefully withdraw the syringe and press the blood collection site with dry cotton wool to stop bleeding quickly.

5. Mix thoroughly

The anticoagulant must be fully mixed with the blood to exert its anticoagulant effect. Uneven mixing often results in blood clots, which affects the next step of use. After mixing, the anticoagulated blood can be pushed into other test containers only after the syringe needle is removed.

6. Vacuum blood collection tube

Laboratories with the conditions can directly purchase vacuum blood collection tubes, which contain anticoagulants and are under negative pressure. After the connected needle successfully pierces the blood vessel, blood automatically flows into the tube. No syringe is required, which is convenient and quick.

3. Notes

It is best to filter and sterilize the anticoagulant after preparation, and the storage time should not be too long; the anticoagulant and blood should be fully mixed to avoid the formation of blood clots!