The efficacy and function of ovalbumin

When it comes to ovalbumin, many people don't know much about it, but it has many functions for the human body, including the treatment of shock caused by excessive blood loss, hypoproteinemia, etc. It also has very good effects in the treatment of neonatal hyperbilirubinemia. With the development of science and technology, ovalbumin is used more and more widely in medicine, and plays an increasingly important role in people's lives.

1. Function

Shock caused by blood loss, trauma, and burns. 2. Increased intracranial pressure caused by cerebral edema and injury. 3. Edema or ascites caused by cirrhosis and kidney disease. 4. Prevention and treatment of hypoproteinemia. 5. Neonatal hyperbilirubinemia. 6. Used for cardiopulmonary bypass, adjuvant treatment of burns, adjuvant treatment of hemodialysis and adult respiratory distress syndrome.

2. Determination of Ovalbumin

Given that N-ovalbumin will still spontaneously transform into S-ovalbumin at room temperature and the transformation is irreversible[11], how to quickly detect S-ovalbumin is an urgent problem to be solved.

The determination of S-ovalbumin content is usually carried out by diluting egg white (or protein solution) and then heating it in a water bath [6]: a portion of the egg white dilution is transferred into a sealed test tube, which is heated in a water bath for a period of time and then cooled. Then add the precipitant, stir evenly, and pour into the centrifuge tube; in addition, rinse the test tube with the same amount of precipitant to reduce liquid loss. Mix the contents of the centrifuge tube, let it stand for a while, centrifuge it at high speed for a few minutes, remove part of the supernatant, and mix it with double the amount of biuret solution. After standing for a while at room temperature, the absorbance at 540 nm was measured. The control sample is a mixture of deionized water with the same amount as the supernatant and double the amount of biuret solution.

There is also a method for determining S-ovalbumin by diluting egg white (or protein solution) and heating it in a water bath. Due to the obvious difference in thermal stability between ovalbumin and S-ovalbumin, natural ovalbumin denatures and forms a precipitate when heated, while S-ovalbumin, which has a significantly better thermal stability than natural ovalbumin, has a certain heat resistance and remains in its original state at the same temperature, remaining in the supernatant. Biuret solution is added to the supernatant and the S-ovalbumin content is calculated based on the absorbance. The essence of the biuret method is to react with the peptide bonds between amino acid residues in proteins, so some short peptide chains will also show a certain absorbance.

In order to address some drawbacks, molecular imprinting technology was used to perform specific molecular recognition on S-ovalbumin in the supernatant to determine its content. Molecular imprinting technology is widely used in the identification of small molecules, and some scholars have studied the molecular imprinting of albumin in egg white. Molecular imprinting polymers are made based on template molecules and can specifically identify target molecules. They have the characteristics of high resolution, good stability and long service life.