What diseases can high levels of leucyl aminopeptidase cause?

Leucyl aminopeptidase is a kind of tissue inside the human body. If the level of leucyl aminopeptidase is too high, it will easily cause some physical diseases, such as hepatitis or liver blockage. It will also easily lead to liver cancer and bile duct cancer. It is very harmful to the health of the body and requires detailed examination and treatment as soon as possible.

What diseases can high levels of leucyl aminopeptidase cause?

Leucyl aminopeptidase is a protease that is abundant in the liver. When there is extrahepatic or extrahepatic bile stasis, LAP activity increases significantly, especially in malignant bile stasis, and its activity continues to increase with the progression of the disease. The reagent is valuable for the diagnosis of hepatic tract obstruction and pancreatic cancer. Liver gangrene, liver tumors, hepatitis, breast cancer, liver cancer, bile duct cancer, pancreatic cancer, endometrial cancer, and ovarian cancer have increased significantly. Cirrhosis and infectious hepatitis may increase moderately, often 2-4 times the reference value. Obstructive jaundice increases significantly, often reaching more than 5 times the reference value, and appears before the increase of bilirubin or ALP.

Unlike other liver function enzymes, which can only be tested in blood samples, LAP can also be tested in urine. In some cases, changes in LAP can be detected in the urine without the need for a blood sample. Leucine aminopeptidase (LAP) LAP is widely present in various tissues of the human body. When toxic substances or diseases affect the proximal tubules rich in LAP, the activity of urinary LAP is highest. LAP increases when the permeability of the glomerular basement membrane increases, tubular epithelial cells are damaged, drug-induced toxic nephropathy occurs, and renal tumors occur. Increased urinary LAP after tumor treatment indicates tumor recurrence. An analysis of various renal cases revealed that LAP had the highest positivity rate.

Clinical significance

Abnormal results: This enzyme is widely distributed in various tissues of the human body, with the highest content in the liver, pancreas, gallbladder, kidney, small intestine and uterine muscle layer. The activity of this enzyme increases in pancreatic cancer, with a positive rate of 100%. When the activity of this enzyme is normal, pancreatic cancer can be ruled out, but the activity of this enzyme may also increase in hepatobiliary diseases. Therefore, the clinical application value of this enzyme is similar to that of ALP, but the activity of this enzyme is normal in bone cancer. In addition, the activity of this enzyme gradually increases after 2 months of normal pregnancy and then decreases after delivery. This point should be taken into account when judging the results.

Urinary LAP activity is highest when toxic substances or diseases affect the proximal tubules, which are rich in LAP. LAP increases when the permeability of the glomerular basement membrane increases, tubular epithelial cells are damaged, drug-induced toxic nephropathy occurs, and renal tumors occur. Increased urinary LAP after tumor treatment indicates tumor recurrence. An analysis of various renal cases revealed that LAP had the highest positivity rate.

People who need to be examined: Auxiliary diagnosis of liver and gallbladder diseases or analysis of kidney damage.

Precautions

Unsuitable groups: Generally no special groups: Taboos before examination: Eat normally and be mentally prepared for the examination.

Inspection process

Dimethylaminobenzaldehyde colorimetric method:

(1) When the enzyme activity exceeds 400u/L, dilute the sample and retest.

(2) The Km obtained by this method is 8.8×10-2mmol/L. The substrate concentration in the enzyme reaction solution of this method is 1.28mmol/L, which is about 15 times the Km value.