How to check the effectiveness of blocking antibodies

Blocking antibodies should be scientifically examined, because after paying attention to the examination and understanding the situation, effective treatment methods can be adopted. Common examinations include APLA examinations. In clinical practice, patients should pay attention to communicating more with doctors and let doctors understand their medical history.

1. Inspection

For patients with recurrent spontaneous abortion, APLA examination is performed first. If APLA is negative, active immunotherapy with the husband's lymphocytes is used. The specific course of treatment is 3-4 injections before pregnancy, and APLA recheck. If APLA turns positive, conception can be planned (including ovulation monitoring, ovulation induction, artificial insemination, in vitro fertilization, etc.). If APLA is still negative, injection treatment must be continued until APLA turns positive before considering conception. After pregnancy, injection treatment should be strengthened for 3 times. A large number of studies have confirmed that lymphocyte immunotherapy for RSA is effective and safe, and APLA has a good predictive value for the outcome of subsequent pregnancy.

2. Treatment methods

For those who lack blocking antibodies and anti-idiotypic antibodies, the main method used is spouse's leukocyte immunotherapy, which can increase the level of blocking antibodies in patients with recurrent spontaneous abortion. After the antibodies rise, pregnancy can be arranged to ensure successful pregnancy. During the treatment, medical staff drew a certain amount of peripheral blood from the husband for centrifugal sedimentation to separate and culture lymphocytes, and then infused them back into the skin of his wife's forearm. One cycle is done every 4 weeks, and this continues until the wife is four or five months pregnant to ensure that she has a normal pregnancy!

3. Treatment process

1. In clinical practice, the first thing to do is to communicate and understand the patient more comprehensively, ensure that both husband and wife agree to undergo blocking antibody testing, and also have a certain understanding of the patient's medical history.

2. Before deciding to start treatment, men need to check whether they have any infectious diseases. If they are not infectious, they can undergo corresponding immune tests. Women need to avoid the menstrual period when undergoing the test.

3. After drawing the corresponding blood from men, they need to go through a sterile operation to separate the lymphocytes in the blood and make appropriate adjustments. The extracted lymphocytes can be used for immunotherapy in women.

4. After treatment, if the result shows that the blocking antibody is still negative, then women need to continue treatment. If it has turned positive, then they can consider trying to get pregnant, and they will need to continue treatment after pregnancy. During the treatment period, female friends should avoid sexual intercourse to avoid affecting the effect of treatment.