What is CST antibody

I believe many people don’t know what CST antibody is, or have never even heard of this term. But don’t worry, knowledge is accumulated! Today we will introduce to you the relevant knowledge about CST antibodies. I believe that after reading the introduction carefully, you will no longer be unfamiliar with CST antibodies. Let’s take a look together below.

The reference antibodies are as follows

1. Bax and western, of course it is better to do immunohistochemistry, I don't know if it is more expensive to do both, there are these types on the CST website,

No. Name Applications Reactivity No.

Name

Applications

Reactivity

5023

Bax (D2E11) Rabbit mAb

W IP IHC-PH

2772

Bax Antibody

W IP HMR Mk

2774

Bax Antibody (Human Specific)

W IP IHC-PH Mk

I don't know what the reactivity at the end means. I am working with human gastric cancer cell lines and leukemia cell lines.

2. Bcl-2, same requirements as before.

2870

Bcl-2 (50E3) Rabbit mAb

W IP HMR (Mk) (C) (B) (Dg)

3498

Bcl-2 (D17C4) Rabbit mAb (Mouse Preferred)

W IP HM

4223

Bcl-2 (D55G8) Rabbit mAb (Human Specific)

W I P H

2876

Bcl-2 Antibody

W H

2872

Bcl-2 Antibody (Human Specific)

W H

What do the words mouse prefered and human specific mean after these?

3.Actin, western

4970

β-Actin (13E5) Rabbit mAb

W IHC-P IHC-F

IF-IC F

HMR Mk B Pg (C) (Dg) (Hr)

5057

β-Actin (13E5) Rabbit mAb

(Biotinylated)

WF HMR Mk B Pg (C) (Hr)

5125

β-Actin (13E5) Rabbit mAb

(HRP Conjugate)

W HMR Mk B Pg (C) (Hr)

8457

β-Actin (D6A8) Rabbit mAb

W IF-IC HMR

3700

β-Actin (8H10D10) Mouse mAb

W IHC-P IF-IC

F

HMR Hm Mk

4967

β-Actin Antibody

W

HMR Hm Mk Mi Dm ZB (C) (X) (Dg) (Pg) (Hr)

Actin mainly looks at which quotation is cheaper and more stable. It would be better if there is a recommendation. It doesn't have to be from CST, other internal references are also fine.

How to store CST antibodies?

A: Please store the original tube of antibody at -20°C and do not aliquot. Because the antibodies are stored in a buffer containing 50% glycerol, they remain liquid at -20°C and will not degrade due to repeated freezing and thawing. Please refer to the product instructions for specific storage temperature.

2. Question: How to store SDS-lysed phosphorylated protein samples?

A: If used in the short term (less than 3 months), SDS sample cell lysate can be stored at -20°C. If stored long term, it should be stored at -80°C. The degradation of activated protein samples varies greatly, with the rate of degradation depending on the nature of each protein and how much activated protein is present in the sample.

3. Question: Can I incubate the antibody at room temperature for 1 hour instead of overnight at 4°C?

Answer: Not recommended. CST's activation state-specific antibodies are designed to distinguish between one or two post-translationally modified amino acid residues. These modifications include phosphorylation, acetylation and enzymatic cleavage. Most antibodies directed against these modification sites have lower affinities compared to antibodies recognizing the total protein. Therefore, it is critical to prolong the reaction time of antigen and antibody by incubating at 4°C overnight to obtain the best signal-to-noise ratio.

After reading the above introduction about CST antibodies, I don’t know how much you know about CST antibodies, but I believe that you will no longer be ignorant of CST antibodies. Of course, if you are interested, you can also go to professional websites and libraries to search for more knowledge. Friends who have the conditions can also consider asking professionals for advice.