Although now is not a period when AIDS is spreading wildly, it is inevitable that we may get AIDS due to the misuse of other people's things or accidental infection. AIDS is like a virus, invisible and intangible. If you want to rule out AIDS, you need to conduct laboratory tests. If the patient's test result is negative, it can be ruled out. If it is positive or negative, repeated confirmation is required. Laboratory Tests for AIDS The laboratory test results of human acquired immunodeficiency virus (HIV) are an important objective basis for the diagnosis, management and treatment of HIV-infected people and AIDS. The application of new methods and technologies in clinical laboratories provides hematological, immunological, serological and etiological laboratory indicators for the early diagnosis of HIV. Currently, laboratory tests mainly include: 1. Virus detection, including HIVp24 antigen detection, HIV nucleic acid determination (viral load detection), and HIV virus isolation and culture. 2. Detect the antiviral antibodies produced in HIV-infected people, that is, HIV antibody test. 3. Detect the immune function of HIV-infected people, mainly CD4T, CD8T and CD4T/CD8T. 4. Other auxiliary examinations include blood cell count, liver and kidney function and other serological indicators. Confirmation test method for HIV infection When the blood passes the initial screening and the result shows HIV antibody (+), it means that the sample blood is highly suspected of HIV infection. For final confirmation, a Western-Blot (WB) test is required. This test needs to be completed in an AIDS testing confirmation laboratory and a test report will be issued. The Western-blot test is currently the preferred method for confirming HIV antibodies in China. This experiment can identify or confirm the results of the initial screening test. It has high sensitivity and strong specificity. The results are also easy to judge intuitively, and an intuitive positive or negative effect is obtained. Objective basis for diagnosing AIDS According to the diagnostic criteria for HIV/AIDS formulated by my country in 1966, the laboratory basis for the clinical classification of acute HIV infection, asymptomatic HIV infection and AIDS mainly comes from epidemiological history, clinical manifestations and laboratory tests. The laboratory basis for diagnosing AIDS is: 1. HIV antibody positive confirmed by confirmation test. 2. HIVp24 antigen positive. 3. The total number of CD4T lymphocytes is less than 200/mm3. 4.CD4/CD8<1. 5. Decrease in white blood cells and hemoglobin. 6. Increased a2 microglobulin levels. 7. Pathological evidence of the pathogens or tumors of the above-mentioned combined infections can be found. When the patient has an epidemiological history of AIDS, any one of the clinical manifestations, and items 1, 3, and 7 of the laboratory tests, the case can be confirmed as AIDS. |
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